منابع مشابه
Dengue virus replicative intermediate RNA detection by reverse transcription-PCR.
Dengue virus replication involves synthesis of a replicative intermediate RNA (RI-RNA), whose presence reveals an actual infection. We report on a simple and rapid reverse transcription-PCR for the detection of viral RI-RNA in infected cells. The product is demonstrated at 20 min postinfection. This method is useful for the study of virus-cell tropism.
متن کاملReplication of bacteriophage ribonucleic acid: analysis of the ultrastructure of the replicative form and the replicative intermediate of bacteriophage R17.
A detailed qualitative and quantitative comparison was made of the ultrastructure of single-stranded ribonucleic acid (RNA) from bacteriophage R17 and double-stranded replicative form (RF) and replicative intermediate (RI) from cells infected with this bacteriophage. The nucleic acids were prepared for electron microscopy by the protein monolayer spreading technique of Kleinschmidt. Single-stra...
متن کاملThe investigation of an arbovirus encephalitis.
A definition of an arbovirus and a broad idea of the groups and the numbers isolated and causing human infection are given. The small incidence of clinical disease compared with overall infection rates is stressed. The conditions for the successful survival of arboviruses is outlined. The investigation of the illness and origin of infection is described. The role of viral antibody in the develo...
متن کاملA haemocyte tropism for an arbovirus.
Horizontally transmitted mosquito-borne viruses enter the midgut with a blood meal then disseminate to infect the salivary glands. En route to the salivary glands, these viruses encounter the plasma (haemolymph) and blood cells (haemocytes). Haemocytes respond to a variety of micro-organisms, but their role in virus replication and dissemination has not been described. To look for a potential h...
متن کاملPurification and properties of the replicative intermediate of the RNA bacteriophage R17.
24 Brimacombe, R., J. Trupin, M. Nirenberg, P. Leder, M. Bernfield, and T. Jaouni, these PROCEEDINGS, 54, 954 (1965). 25 S61, D., E. Ohtsuka, D. S. Jones, R. Lohrmann, H. Hayatsu, S. Nishimura, and H. G. Khorana, these PROCEEDINGS, 54, 1378 (1965). 26Brenner, S., A. 0. W. Stretton, and S. Kaplan, Nature, 206, 994 (1965). 27 Weigert, M. G., and A. Garen, Nature, 206, 992 (1965). 28Ambler, R. P.,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1968
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.2.6.547-552.1968